Rewiring the streptomyces cell factory for cost-effective production of biomolecules

  • Description
  • Details
  • Subprojects
  • History
  • Relations
  • Publications
Project Title: Rewiring the streptomyces cell factory for cost-effective production of biomolecules
Project Number: CORDIS-111042
Project web address: Follow on CORDIS
Organization: Katholieke Universiteit Leuven, Belgium, Leuven
Collaborators: Technion-Israel Institute Of Technology, IL
University Of Manchester, GB
Technische Universitaet Berlin, DE
University Of Bielefeld, DE
Foundation For Research And Technology Hellas, GR
Forschungszentrum Jülich Gmbh, DE
Universitat Wien, AT
Norges Teknisk-Naturvitenskapelige Universitet Ntnu, NO
Helmholtz-Zentrum Fuer Infektionsforschung Gmbh, DE
Matis Ohf, IS
Entrechem Sl, ES
Ustav Molekularnej Biologie Slovenskej Akademie Vied, SK
Apronex S.R.O, CZ
Q-Biologicals Nv, BE
Pharmabiotec Gmbh, DE
Progenus Sa, BE
Principal Investigators (PI): Liz Fay, GB
Myriam Witvrouw, BE
Iris Litty, DE
Jack Lavan, IL
Michael Straetz, DE
Claudia Neuhs, DE
Andreas Plagakis, GR
Ladislav Andera, CZ
Francisco Moris, ES
Jacqueline Bock, DE
Stefan De Graaf, NO
Annie Van Broekhoven, BE
Kristinn Kolbeinsson, IS
Jan Kormanec, SK
Sergey Zotchev, AT
Rudolf Guggenmoser, DE
Robert Renaville, BE
 
Project Description:
STREPSYNTH aims to set-up a Streptomyces-based new industrial production platform (SNIP) for high value added biomolecules. Streptomyces lividans was chosen as a bacterial host cell because it has been already shown to be highly efficient for the extracellular production of a number of heterologous molecules that vary chemically, has a robust tradition of industrial fermentation and is fully accessible to genetic intervention. To develop SNIP our strategy has two components: first, we will construct a collection of reduced-genome S. lividans strains. This will metabolically streamline the cell and rid it of agents (e.g. proteases) of potential harm to the heterologous polypeptides. Second, we will engineer synthetic parts and cassettes, i.e. reshuffled, rewired and repurposed genetic elements either indigenous to S. lividans or heterologous genes organized in artificial operon clusters. These elements will serve three aims: transcriptional and translational optimization, sophisticated on-demand transcriptional regulation that will provide unique fermentation control and metabolic engineering of complete cellular pathways channeling biomolecules to profuse extracellular secretion. Synthetic parts and cassettes will be either directly incorporated into the genome or be hosted in the form of plasmids. Systems biology tools will guide fine-tuning rounds of cell factory engineering and fermentation optimization. To set up SNIP we chose two classes of biomolecules with obvious immediate industrial value and application: heterologous proteins (industrial enzymes, biopharmaceuticals, biofuel enzymes, diagnostics) and small molecules (lantipeptides and indolocarbozoles) useful for multiple industrial purposes (biopharmaceuticals, additives, food technology, bioenergy). These biomolecules are of immediate interest to SMEs that participate and guide the industrial relevance of STREPSYNTH. SNIP is a modular platform that can be repurposed for diverse future applications.
 
Project Terms:
agriculture biotechnology environmental protection scientific research
Project Title: Rewiring the streptomyces cell factory for cost-effective production of biomolecules
Project Number: CORDIS-111042
Project web address: Follow on CORDIS
Organization: Katholieke Universiteit Leuven, Belgium, Leuven
Collaborators: Technion-Israel Institute Of Technology, IL
University Of Manchester, GB
Technische Universitaet Berlin, DE
University Of Bielefeld, DE
Foundation For Research And Technology Hellas, GR
Forschungszentrum Jülich Gmbh, DE
Universitat Wien, AT
Norges Teknisk-Naturvitenskapelige Universitet Ntnu, NO
Helmholtz-Zentrum Fuer Infektionsforschung Gmbh, DE
Matis Ohf, IS
Entrechem Sl, ES
Ustav Molekularnej Biologie Slovenskej Akademie Vied, SK
Apronex S.R.O, CZ
Q-Biologicals Nv, BE
Pharmabiotec Gmbh, DE
Progenus Sa, BE
Principal Investigators (PI): Liz Fay, GB
Myriam Witvrouw, BE
Iris Litty, DE
Jack Lavan, IL
Michael Straetz, DE
Claudia Neuhs, DE
Andreas Plagakis, GR
Ladislav Andera, CZ
Francisco Moris, ES
Jacqueline Bock, DE
Stefan De Graaf, NO
Annie Van Broekhoven, BE
Kristinn Kolbeinsson, IS
Jan Kormanec, SK
Sergey Zotchev, AT
Rudolf Guggenmoser, DE
Robert Renaville, BE
 
Project Categories:
Natural Sciences
 
Other Information:
Fiscal Year: 2013
Project Start Date: 1 December 2013
Project End Date: 30 November 2018
Project program: FP7-KBBE
 
Project Funding Information:
Funding Mechanism: CP-TP - Collaborative Project targeted to a special group (such as SMEs)
Year Funding Organization Total Funding, $
2013 European Research Council $12,463,462